Traditionally Smithia sensitiva is used in inflammation and in cancer. Our study objective is to isolate and characterize the phytoconstituent present in the plant Smithia sensitiva. Qualitative chemical analysis of the extract of Smithia sensitiva revealed the presence of alkaloids, glucose and carbohydrates, steroids, tannins and flavanoids. The plant was extracted using different solvents and isolated different constituents present in the crude extract by centrifugation and their purification by column chromatography. The ethyl acetate: methanol (15:85) soluble fraction of the compound was crystallized as yellow crystals and it was purified by column chromatography using the same ratio and the eluted portion was found to be a single compound in the TLC. The spectral information indicate that the molecule was 5, 6 dihydroxy7, 8, 3’trimethoxy flavone D-glucose-D-xylose. The structure of the compound was confirmed by I.R, Mass, C13 NMR and H1 NMR studies.
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